Plan Aim: The aim of this audition is to identify the optimum pH for which the enzyme, amylase, workings best at. Hypothesis: I annunciate that enzyme bodily process will decrease if it steers away from 6-8 pH as this is the optimum pH start out for which the enzyme amylase works best. Variables: Dependant Variables: The time it takes the enzyme to react. maintain Variables: The summate of starch put in The join of enzyme solution The amount of Iodine solution The amount of water in ML The time taken for the reaction. separatist Variables: The range of pH, between 6-8 pH. Method: 1. Firstly, mooring the buffer literal contraceptives into a test-tube containing 20 ml of water (at room temperature). 2. formerly the buffer tablet has dissolved into the water, add 10 ml of starch solution. 3. later the starch solution has been added, add 10 ml of enzyme solution to the current solution, simultaneously start the timepiece and record at 1 minute intervals. 4. In addition, pip iodine in the dropping tile in birth to identify if starch is currently present. 5. Repeat each experiment 3 times. Equipment: 1. drop Tile 2. Pipette 3. Test underpass 4. Starch ancestor 5. Iodine Solution 6. Enzyme Solution 7. Beaker(s) 8. weewee 9. Measuring Cylinder (ml) 10. oecumenical Indicator 11.
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